Cell culture
Alexander Röntgen
Published: 2023-11-02 DOI: 10.17504/protocols.io.36wgq3bk5lk5/v1
Abstract
Protocol to culture SH-SY5Y cells.
Steps
Cell culture
1.
Culture human SH-SH5Y cells at 37 °C and 5% CO2
2.
Split cells at 80–90% confluency
2.1.
Discard medium, rinse with PBS
2.2.
Incubate cells with Trypsin-EDTA for 5 min at 37 °C and 5% CO2
2.3.
Add 9 mL medium to neutralise Trypsin-EDTA
2.4.
Centrifuge at 300 g , 5 min, RT
2.5.
Discard medium
2.6.
Resuspend in 10 mL medium
3.
Split at desired ratio into fresh medium
