Affinity-based profiling of endogenous phosphoprotein phosphatases by mass spectrometry

Brooke L. Brauer, Kwame Wiredu, Sierra Mitchell, Greg B. Moorhead, Scott A. Gerber, Arminja N. Kettenbach

Published: 2021-09-12 DOI: 10.1038/s41596-021-00604-3

Supplementary information

Annotated PPP subunits and interactors. Uniprot ID and entry name are given for each protein, alongside its gene name and description. PPP subunit indicates the PPP the proteins belong to (PP1-7), whether it is a catalytic subunit (C), a regulatory subunit (R), an inhibitory protein (i), or an activating protein (a).

Supplementary Video 1

A step-by-step demonstration of making a StageTip and using it to desalt a TMT label check sample.

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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Supplementary information

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Affinity-based profiling of endogenous phosphoprotein phosphatases by mass spectrometry

Supplementary information

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