Advances in mass spectrometry to unravel the structure and function of protein condensates Cagla Sahin, Axel Leppert, Michael Landreh Published: 2023-10-30 DOI: 10.1038/s41596-023-00900-0 Mass Spectrometry Protein Condensates Liquid-Liquid Phase Separation Amyloids Microenvironments AI 解读 浏览 1208 PDFMetadataSupplementary information Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Self-assembled ultraflexible probes for long-term neural recordings and neuromodulation iDRiP for the systematic discovery of proteins bound directly to noncoding RNA A robust unsupervised machine-learning method to quantify the morphological heterogeneity of cells and nuclei Spooling electrochemiluminescence spectroscopy: development, applications and beyond Acoustic tweezers for high-throughput single-cell analysis A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues An integrated pipeline for comprehensive analysis of immune cells in human brain tumor clinical samples Purification and functional characterization of novel human skeletal stem cell lineages Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors 查看全部 Seahorse protocol for islets using Xfe24 Analyzer Quantification of synapse polarization ("the conjugate assay") Anti-Borrelia ELISA (IgG) A protocol for a scoping review of implementation strategies to scale up self-administered depot medroxyprogesterone acetate subcutaneous injectable contraception Scoping review; implementation strategy; scale up; self administration; depot medroxyprogesterone acetate subcutaneous injectable; contraception QIAGEN QIAseq FX Protocol metagenomics Nanopore sequencing WGS Automated Protein Quantification with the Biomek-FX Liquid Handler System Protein quantification Automation Biomek Lowry assay Proteomics Sample preparation FLASH-seq protocol FLASH-Seq single-cell RNA-sequencing full-length tagmentation Labelling kelps with 13C and 15N for isotope tracing or enrichment experiments Macroalgae Kelp Seaweed Cultivation Isotopic enrichment Isotope tracing experiments Enrichment experiments Total and PS129 aSyn levels using Western Blot ASAPCRN alpha-syuclein Western blot 2,4-dinitrophenylhydrazine alpha-ketoglutarate detection assay for Prolyl Hydroxylase Domain (PHD) proteins 查看全部 New Histone Lysine Acylation Biomarkers and Their Roles in Epigenetic Regulation acetylation epigenetics histone lysine acylation metabolism Models of Affective Illness: Chronic Mild Stress in the Rat animal model antidepressant treatments chronic stress depression treatment-resistant depression Differentiation and Subculturing of Renal Proximal Tubular-like Cells Derived from Human iPSC differentiation induced pluripotent stem cells proximal tubular-like cells subculturing transwell Accurate and Effective Detection of Recurrent Copy Number Variants in Large SNP Genotype Datasets bioinformatics pipeline CNVs structural variation SNPs Influence of Admixture on Phenotypes admixture mapping differential allele frequency linkage disequilibrium phenotype Mammalian Chromosome Analysis and Sorting by Flow Cytometry flow cytogenetics isolation protocols karyotype mammalian chromosomes sorting The MegaPool Approach to Characterize Adaptive CD4+ and CD8+ T Cell Responses AIM epitopes infectious diseases lyophilization SARS-CoV-2 T cell Dissecting the Biology of the Fungal Wheat Pathogen Zymoseptoria tritici: A Laboratory Workflow fungal isolation in vitro growth Septoria tritici blotch virulence assay wheat pathogen Autologous Induced Pluripotent Stem Cell–Based Cell Therapies: Promise, Progress, and Challenges autologous cell therapies cell therapy induced pluripotent stem cells (iPSCs) regenerative medicine DIAMOND+MEGAN: Fast and Easy Taxonomic and Functional Analysis of Short and Long Microbiome Sequences functional binning metagenome assembled genomes microbiome sequencing protein alignment software taxonomic binning 查看全部