Acid Wash Protocol (Alegado Lab)
Ashleyolguin
Published: 2023-03-20 DOI: 10.17504/protocols.io.14egn213qg5d/v1
Abstract
This protocol guides lab members through the acid wash process
Steps
Pre-requisites (BEFORE proceeding to acid washing)
1.
3.
Gather bottles to be washed and take over to acid washing lab
Acid Washing
4.
Fill bin with 10% Hydrochloric acid solution about ½ to 2/3 full and submerge bottles in bin.
5.
Fill bottles with acid solution, cap and place in bin.
6.
Cover bin with lid.
--> Note: insure cover is secured.
7.
Let sit for minimal of 2 hours.
--> Note: place bin on Acid wash rack.
10.
Empty bin and rinse with miliQ water.
11.
Dry
Neutralization of Acid
12.
Plug the drain and test the seal using water
13.
Use acid rinse until the sink fills 3/4 maximum
14.
Add sodium bicarbonate