A ‘print–pause–print’ protocol for 3D printing microfluidics using multimaterial stereolithography

Yong Tae Kim, Alireza Ahmadianyazdi, Albert Folch

Published: 2023-01-05 DOI: 10.1038/s41596-022-00792-6

3D printing

microfluidics

stereolithography

multimaterial chips

biocompatible resins

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Supplementary information

Supplementary Information

Supplementary Figs. 1–3.

Reporting Summary

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Supplementary information

Folch, A. Introduction to BioMEMS (CRC Press, 2012).
Liu, P. & Mathies, R. A. Integrated microfluidic systems for high-performance genetic analysis. Trends Biotechnol. 27, 572–581 (2009).
Duncombe, T. A., Tentori, A. M. & Herr, A. E. Microfluidics: reframing biological enquiry. Nat. Rev. Mol. Cell Biol. 16, 554–567 (2015).
Elvira, K. S., Casadevall i Solvas, X., Wootton, R. C. R. & DeMello, A. J. The past, present and potential for microfluidic reactor technology in chemical synthesis. Nat. Chem. 5, 905–915 (2013).
Whitesides, G. M., Ostuni, E., Takayama, S., Jiang, X. & Ingber, D. E. Soft lithography in biology and biochemistry. Annu. Rev. Biomed. Eng. 3, 335–373 (2003).
Weibel, D. B., Diluzio, W. R. & Whitesides, G. M. Microfabrication meets microbiology. Nat. Rev. Microbiol. 5, 209–218 (2007).
Hsu, C.-H. & Folch, A. Spatio-temporally-complex concentration profiles using a tunable chaotic micromixer. Appl. Phys. Lett. 89, 144102 (2006).
Lam, E. W., Cooksey, G. A., Finlayson, B. A. & Folch, A. Microfluidic circuits with tunable flow resistances. Appl. Phys. Lett. 89, 164105 (2006).
Au, A. K., Huynh, W., Horowitz, L. F. & Folch, A. 3D-printed microfluidics. Angew. Chem. Int. Ed. 55, 3862–3881 (2016).
Bhattacharjee, N., Urrios, A., Kang, S. & Folch, A. The upcoming 3D-printing revolution in microfluidics. Lab Chip 16, 1720–1742 (2016).
Nielsen, A. V., Beauchamp, M. J., Nordin, G. P. & Woolley, A. T. 3D printed microfluidics. Annu. Rev. Anal. Chem. 13, 45–65 (2020).
Naderi, A., Bhattacharjee, N. & Folch, A. Digital manufacturing for microfluidics. Annu. Rev. Biomed. Eng. 21, 325–364 (2019).
Urrios, A. et al. 3D-printing of transparent bio-microfluidic devices in PEG-DA. Lab Chip 16, 2287–2294 (2016).
Kim, Y. T., Castro, K., Bhattacharjee, N. & Folch, A. Digital manufacturing of selective porous barriers in microchannels using multi-material stereolithography. Micromachines 9, 125 (2018).
Gong, H., Beauchamp, M., Perry, S., Woolley, A. T. & Nordin, G. P. Optical approach to resin formulation for 3D printed microfluidics. RSC Adv. 5, 106621 (2015).
Gong, H., Woolley, A. T. & Nordin, G. P. High density 3D printed microfluidic valves, pumps, and multiplexers. Lab Chip 16, 2450–2458 (2016).
Lee, M. P. et al. Development of a 3D printer using scanning projection stereolithography. Sci. Rep. 5, 9875 (2015).
Kuo, A. P. et al. High-precision stereolithography of biomicrofluidic devices. Adv. Mater. Technol. 4, 1800395 (2019).
Warr, C. et al. Biocompatible PEGDA resin for 3D printing. ACS Appl. Bio. Mater. 3, 2239–2244 (2020).
Choi, N. W. et al. Microfluidic scaffolds for tissue engineering. Nat. Mater. 6, 908–915 (2007).
Yu, Y. J. et al. Hydrogel-incorporating unit in a well: 3D cell culture for high-throughput analysis. Lab Chip 18, 2604–2613 (2018).
LaVan, D. A., McGuire, T. & Langer, R. Small-scale systems for in vivo drug delivery. Nat. Biotech. 21, 1184–1191 (2003).
Zhang, X., Li, L. & Luo, C. Gel integration for microfluidic applications. Lab Chip 16, 1757–1776 (2016).
Cuchiara, M. P., Allen, A. C. B., Chen, T. M., Miller, J. S. & West, J. L. Multilayer microfluidic PEGDA hydrogels. Biomaterials 31, 5491–5497 (2010).
Sip, C. G., Bhattacharjee, N. & Folch, A. Microfluidic transwell inserts for generation of tissue culture-friendly gradients in well plates. Lab Chip 14, 302–314 (2013).
Cate, D. M., Sip, C. G. & Folch, A. A microfluidic platform for generation of sharp gradients in open-access culture. Biomicrofluidics 4, 044105 (2010).
Colosi, C. et al. Microfluidic bioprinting of heterogeneous 3D tissue constructs using low-viscosity bioink. Adv. Mater. 28, 677–684 (2016).
Kang, E. et al. Digitally tunable physicochemical coding of material composition and topography in continuous microfibres. Nat. Mater. 10, 877–883 (2011).
Wehner, M. et al. An integrated design and fabrication strategy for entirely soft, autonomous robots. Nature 536, 451–455 (2016).
Hardin, J. O., Ober, T. J., Valentine, A. D. & Lewis, J. A. Microfluidic printheads for multimaterial 3D printing of viscoelastic inks. Adv. Mater. 27, 3279–3284 (2015).
Skylar-Scott, M. A., Mueller, J., Visser, C. W. & Lewis, J. A. Voxelated soft matter via multimaterial multinozzle 3D printing. Nature 575, 330–335 (2019).
Lind, J. U. et al. Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing. Nat. Mater. 16, 303–308 (2017).
Johnson, B. N. et al. 3D printed anatomical nerve regeneration pathways. Adv. Funct. Mater. 25, 6205–6217 (2015).
Park, S. H. et al. 3D printed polymer photodetectors. Adv. Mater. 30, 1803980 (2018).
Liu, W. et al. Rapid continuous multimaterial extrusion bioprinting. Adv. Mater. 29, 1604630 (2017).
Choi, J. W., Kim, H. C. & Wicker, R. Multi-material stereolithography. J. Mater. Process. Technol. 211, 318–328 (2011).
Zhou, C., Chen, Y., Yang, Z. & Khoshnevis, B. Digital material fabrication using mask-image-projection-based stereolithography. Rapid Prototyp. J. 19, 153–165 (2013).
Zhang, X., Jiang, X. N. & Sun, C. Micro-stereolithography of polymeric and ceramic microstructures. Sens. Actuators A Phys. 77, 149–156 (1999).
Chan, V., Zorlutuna, P., Jeong, J. H., Kong, H. & Bashir, R. Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation. Lab Chip 10, 2062–2070 (2010).
Gauvin, R. et al. Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography. Biomaterials 33, 3824–3834 (2012).
Gong, H., Bickham, B. P., Woolley, A. T. & Nordin, G. P. Custom 3D printer and resin for 18 μm × 20 μm microfluidic flow channels. Lab Chip 17, 2899–2909 (2017).
Kim, Y. T., Bohjanen, S., Bhattacharjee, N. & Folch, A. Partitioning of hydrogels in 3D-printed microchannels. Lab Chip 19, 3086–3093 (2019).
Sigma-Aldrich Co. LLC, Product information Sigmacote (2016); https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/133/975/sl2pis.pdf
Zhu, F., Friedrich, T., Nugegoda, D., Kaslin, J. & Wlodkowic, D. Assessment of the biocompatibility of three-dimensional-printed polymers using multispecies toxicity tests. Biomicrofluidics 9, 061103 (2015).
Macdonald, N. P. et al. Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays. Lab Chip 16, 291–297 (2016).

A ‘print–pause–print’ protocol for 3D printing microfluidics using multimaterial stereolithography

Supplementary information

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