A ‘print–pause–print’ protocol for 3D printing microfluidics using multimaterial stereolithography Yong Tae Kim, Alireza Ahmadianyazdi, Albert Folch Published: 2023-01-05 DOI: 10.1038/s41596-022-00792-6 3D printing microfluidics stereolithography multimaterial chips biocompatible resins AI 解读 浏览 1182 PDFMetadataSupplementary information Supplementary Information Supplementary Figs. 1–3. Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Preparation of single-cell suspensions of mouse glomeruli for high-throughput analysis cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins Engineering megabase-sized genomic deletions with MACHETE (Molecular Alteration of Chromosomes with Engineered Tandem Elements) Genome-wide pooled CRISPR screening in neurospheres Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures Design and fabrication of wearable electronic textiles using twisted fiber-based threads Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells Scanorama: integrating large and diverse single-cell transcriptomic datasets 查看全部 Concentration and nucleic acid extraction of viruses from aggregated airplane waste wastewater-based epidemiology WBE aggregated airplane waste airplane wastewater wastewater viral concentration viromics metagenomics Enhancing the growth of coral microfragments using the 'Green Film' Husbandry Method microfragmentation microfragment coral stony coral aquarium husbandry aquaculture coral culture DNA extraction from recently fertilised Atlantic salmon embryos for use in microsatellite validation of triploidy DNA extraction DNA quality and quantity assessments Microsatellites validation assessment Egg Tiploid Salmon How to grow wheat in controlled and semi-controlled environment Wheat Germination Transplanting Threshing Vernalisation Preparation of 1M calcium chloride solution (CaCl2) ngm worm c.elegans c elegans elegans caenorhabditis media medium calcium chloride RNA-Stable Isotope Probing Stable isotope probing SIP RNA Ultracentrifugation Density gradient Resuspension and concentration of iron chloride precipitated viruses from seawater for HMW DNA extraction Patch-Seq Recording and Extraction Detailed Protocol Patch-Seq patch seq recording electrophysiology neuron PF0301 patching extraction extracting Confirmation of Gene Knockdown with RT-qPCR ASAPCRN DTI preprocessing, statistical and cluster analysis diffusion tensor imaging 查看全部 Protein Sequence Analysis Using the MPI Bioinformatics Toolkit CLANS cluster analysis HHpred HMM homology profile hidden Markov models sequence comparison sequence similarity searches structure prediction Tackling the Mouse-on-Mouse Problem in Cochlear Immunofluorescence: A Simple Double-Blocking Protocol for Immunofluorescent Labeling of Murine Cochlear Sections with Primary Mouse Antibodies antibodies cochlea histological technique immunofluorescence mouse staining Isolation and Micromass Culturing of Primary Chicken Chondroprogenitor Cells for Cartilage Regeneration cartilage formation chicken embryo chondrogenesis limb bud micromass culture Scaled Isolation of Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles EVs exosomes large-scale preparation MSCs Using CRISPR/ttLbCas12a for in planta Gene Targeting in A. thaliana CRISPR/Cas Cas12a temperature-tolerant Using ConSurf to Detect Functionally Important Regions in RNA ConSurf evolutionary analysis functional regions Rate4Site RNA sequence analysis Spatially Defined Gene Delivery into Native Cells with the Red Light-Controlled OptoAAV Technology AAV localized gene therapy optogenetics single-cell biology viral vector OptoAAV technology Assessing Protein Synthesis and Degradation Rates in Arabidopsis thaliana Using Amino Acid Analysis 13CO2 labeling protein degradation protein synthesis Design and Derivation of Multi-Reporter Pluripotent Stem Cell Lines via CRISPR/Cas9n-Mediated Homology-Directed Repair CRISPR/Cas9n human pluripotent stem cells reporter cell lines transfection of cells Regioselective 2′-Silylation of Purine Ribonucleosides for Phosphoramidite RNA Synthesis 查看全部
