A distal lung organoid model to study interstitial lung disease, viral infection and human lung development

Nadine Schrode, Ivana Matkovic Leko, Remy T. Schneider, Tania A. Thimraj, Daniel Beitler, Hsiao-Yun Liu, Kristin Beaumont, Ya-Wen Chen, Hans-Willem Snoeck

Published: 2023-05-09 DOI: 10.1038/s41596-023-00827-6

human pluripotent stem cells

single-cell RNA sequencing

lung organoids

interstitial lung disease

lung development

AI 解读

Purification of infection-associated macropinosomes by magnetic isolation for proteomic characterization

Hi-CO: 3D genome structure analysis with nucleosome resolution

Practical and concise synthesis of nucleoside analogs

Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal–assisted labeling

Detecting chromosomal interactions in Capture Hi-C data with CHiCAGO and companion tools

Capture of the newly transcribed RNA interactome using click chemistry

Design, assembly, and characterization of membrane-spanning DNA nanopores

Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq

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Organoids have been an exciting advancement in stem cell research. Here we describe a strategy for directed differentiation of human pluripotent stem cells into distal lung organoids. This protocol recapitulates lung development by sequentially specifying
Introduction
Materials
Procedure
Timing
Troubleshooting
Anticipated results
- Reporting summary
- References
References
ACKNOWLEDGMENTS

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A distal lung organoid model to study interstitial lung disease, viral infection and human lung development

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