5N HCl
Jacquelina.Woods
Published: 2021-12-02 DOI: 10.17504/protocols.io.byfsptne
Abstract
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR detection, library prep, genome sequencing, quality control checks, and data submission to NCBI. This method provides a reagent formula required in the rapid concentration of intact viruses from wastewater using a combination of PEG precipitation and ultracentrifugation.
Steps
1.
Measure 14.6mL of deionized or ultrapure water into sterile container.
2. This solution should be prepared under a chemical fume hood.
Slowly add 10.4mL
Safety information
3.
Store at 2-8Room temperature.
