- Published: - DOI: - AI 解读 浏览 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols CRISPR-Combo–mediated orthogonal genome editing and transcriptional activation for plant breeding Profiling human brain vascular cells using single-cell transcriptomics and organoids Luciferase- and HaloTag-based reporter assays to measure small-molecule-induced degradation pathway in living cells Publisher Correction: A reconstituted cell-free assay for the evaluation of the intrinsic activity of purified human ribosomes RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19 Optimized whole-genome sequencing workflow for tumor diagnostics in routine pathology practice Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging Optofluidic Raman-activated cell sorting for targeted genome retrieval or cultivation of microbial cells with specific functions Desynchronizing the sleep–wake cycle from circadian timing to assess their separate contributions to physiology and behaviour and to estimate intrinsic circadian period Imaging and optogenetic modulation of vascular mural cells in the live brain 查看全部 First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368) murine RNAse inhibitor transcription Reverse transcription RT cDNA Pseudorabies Virus (PRV) injection into interscapular brown adipose tissue A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols Calculating multiplicity of infection (MOI) calculate calculation determine estimate establish moi multiplicity of infection phage phages bacteriophage bacteriophages bacteria infection multiplicity math numbers multiply divide colony colonies colony-forming units CFU CFUs plaque plaque-forming unit plaque-forming units unit PFU PFUs culture how to Extraction and ONT MinION Library Preparation of uHMW gDNA extraction gDNA MinION nematode library preparation uHMW gDNA ONT Intracardial perfusion of the African turquoise killifish Perfusion fish killifish N. furzeri Nothobranchius furzeri Plasmid Sequence Analysis from Long Reads Nuclear Extraction from Tissue for FACS ASAPCRN Modular automated bottom-up proteomic sample preparation for high-throughput applications Automation Sample preparation Proteomics Protein quantification Biomek Lowry assay Cell lysis Normalization Cell preparation for scRNA-Seq from diluted bodily fluids 查看全部 Exploring Manually Curated Annotations of Intrinsically Disordered Proteins with DisProt biocuration database DisProt IDPs intrinsically disordered proteins Leveraging AI Advances and Online Tools for Structure-Based Variant Analysis AlphaFold gene variants protein structure structural analysis for non-experts variant analysis Current Strategies of Modeling Human Trophoblast Using Human Pluripotent Stem Cells in vitro extravillous trophoblast human trophoblast stem cell human pluripotent stem cell syncytiotrophoblast trophoblast differentiation Expression and Purification of Human U1-70K (snRNP70) and its BAD Domains Using an E. coli Expression System BAD domain protein solubility RNA splicing snRNP70 U1-70K A Sensitive and Quantitative mKeima Assay for Mitophagy via FACS FACS mitophagy mKeima Parkin PINK1 Super-Resolution Stimulated Raman Scattering Microscopy with Graphical User Interface–Supported A-PoD deconvolution metabolic imaging stimulated Raman scattering super-resolution Preparation of a Convertible Spacer Containing a Disulfide Group for Versatile Functionalization of Oligonucleotides oligonucleotide functionalization oligonucleotide conjugation disulfide linker redox-responsive release Assessing Cell Competition in Human Pluripotent Stem Cell (hPSC) Cultures cell competition culture-acquired genetic variants fitness-sensing assays fluorescent hPSC subline human pluripotent stem cells (hPSCs) single-cell cloning hPSCs Agrobacterium-Mediated Transformation of Setaria viridis, a Model System for Cereals and Bioenergy Crops AGL1 Agrobacterium-mediated transformation Agrobacterium tumefaciens bioenergy crops C4 photosynthesis cereals foxtail millet ME034V-1 model system Setaria viridis transformation An In Vitro Microneutralization Assay for Influenza Virus Serology influenza neutralization microneutralization hemagglutinin HA neuraminidase NA 查看全部
