Von Stosch (VS) enriched seawater medium solution
Anton Kuech
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Abstract
This protocol outlines the steps required to prepare von Stosch (VS) enriched seawater medium modified according to Guiry & Cunningham (1984).
Before start
It is important to follow the order of chemicals added as shown in the protocol.
Steps
Vitamin B12 stock solution
5mg 500mL
Primary solution
In 4L
Pasteurise for 2h 0m 0s at 99°C
1.86g
21.26g
0.139g
0.98g
0.05g
0.1g
20mL Vitamin B12 stock solution (5mg in 500mL
10mg
Storage
Store VS solution in Duran bottles in a cold and dark setting at 10°C .
Equipment
| Value | Label |
|---|---|
| Duran® laboratory bottles, with caps | NAME |
| Glass bottle | TYPE |
| Duran | BRAND |
| Z305197-10EA | SKU |
| http://sigmaaldrich.com | LINK |
| 500 ml | SPECIFICATIONS |
Application
Culturing medium is created by adding 200mL VS to 10L sterile seawater
