U54 SCENT Olink® Target 96

Aliquoting and Day of Preparation

For Aliquoting of EDTA plasma samples: For Aliquoting of EDTA plasma samples :

• Thaw all EDTA plasma samples in a -4°C refrigerator until ready to use.
• Keep Samples in a chilled rack whenever possible.
• Match your parent tubes (Original tubes shipped) with a prelabelled 2mL clarification tube.
• Vortex all samples on a Vortex Genie with a vortex setting of 7-10.
• Transfer samples from the parent tube to the clarification tube.
• In a pre-cooled centrifuge set to -4°C centrifuge all samples for 5 minutes on a setting of 20,000 rcf.
• Carefully remove samples from centrifuge into a chilled rack.
• Transfer liquid from the clarification tube to a prelabelled aliquot tube in desired amounts.
• Using Liquid Nitrogen, freeze back the tubes and place in a -80°C freezer until ready to use.

For EDTA samples on day of use: For EDTA samples on day of use se:

• Thaw all EDTA plasma samples in a -4°C refrigerator until ready to use.

• Keep Samples in a chilled rack whenever possible.

• Vortex all samples on a Vortex Genie with a vortex setting of 7-10.

• In a pre-cooled centrifuge set to -4°C centrifuge all samples for 5 minutes on a setting of 20,000 rcf.

• Return the samples to a chilled rack.

• Your samples are ready for use.

Once samples are prepared use the following links to access the protocols:

• Olink Target 96 Short Instructions
• Olink Target 96 User Manual