TS Procure 812 - primary fixation formalin/wet tissue (TM - 013)
sandra.crameri
Published: 2023-05-09 DOI: 10.17504/protocols.io.bp2l61qbdvqe/v2
Abstract
The method is used to process formalin fixed histological tissues to Procure 812 resin blocks.
Steps
HEADER
1.
SAN:
SPEC No:
OPERATOR & STEPS:
OPERATOR & STEPS:
Primary fixation
2.
Formalin for undefined extended period of time at Room temperature
Conventional
3.
2.5% volume 0.1Molarity (M) Sorenson's Phosphate buffer (pH 7.2, 300mosmol/kg) for at least 0h 40m 0s or overnight
4.
0.1Molarity (M) buffer 0h 15m 0s
5.
1% volume 1h 0m 0s
6.
70% volume 0h 20m 0s or overnight
7.
95% volume 0h 20m 0s
8.
100% volume 0h 20m 0s
9.
100% volume 0h 20m 0s
10.
50% volume 50% volume 0h 20m 0s
11.
100% volume 0h 20m 0s
12.
50% volume 50% volume Procure 812
13.
100% volume Procure 8120h 20m 0s
14.
100% volume Procure 812 1h 0m 0s
15.
Embed & polymerise overnight in oven at 60°C
