TS Procure 812 - primary fixation Karnovsky's - tissue (TM - 013)
sandra.crameri
Published: 2023-05-09 DOI: 10.17504/protocols.io.e6nvwj342lmk/v1
Abstract
The method is used to process conventionally EM fixed 1mm3 tissues to Procure 812 resin blocks.
Steps
HEADER
1.
SAN:
SPEC No:
OPERATOR & STEPS:
OPERATOR & STEPS:
Conventional
2.
Karnovsky's fixative: 2.5% volume 4% volume 0.1Molarity (M) Sorenson's Phosphate buffer (pH 7.2, 300mosmol/kg) for at least 0h 40m 0s or overnight
3.
0.1Molarity (M) buffer 0h 15m 0s
4.
1% volume 1h 0m 0s
5.
70% volume 0h 20m 0s or overnight
6.
95% volume 0h 20m 0s
7.
100% volume 0h 20m 0s
8.
100% volume 0h 20m 0s
9.
50% volume 50% volume 0h 20m 0s
10.
100% volume 0h 20m 0s
11.
50% volume 50% volume Procure 812
12.
100% volume Procure 8120h 20m 0s
13.
100% volume Procure 812 1h 0m 0s
14.
Embed & polymerise overnight in oven at 60°C
