SynBot Protocols

Justin T Savage, Juan Ramirez, Dolores Irala, Cagla Eroglu

Published: 2024-06-24 DOI: 10.17504/protocols.io.3byl4qewjvo5/v2

浏览 1126

Abstract

Protocols related to the paper SynBot: An open-source image analysis software for automated quantification of synapses . Includes details of rat neuron and astrocyte culture, in vitro synapse staining, in vivo synapse staining, and using SynBot with manual or ilastik thresholding.

Steps

推荐阅读

Nature Protocols

Protocols IO

Current Protocols

In vitro reconstitution of COPII vesicles fromArabidopsis thalianasuspension-cultured cells

Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins

Monomicellar assembly to synthesize structured and functional mesoporous carbonaceous nanomaterials

An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells

Measuring DNA modifications with the comet assay: a compendium of protocols

Design and build of small-scale magnetic soft-bodied robots with multimodal locomotion

A practical guide to scanning light-field microscopy with digital adaptive optics

Reproducible mass spectrometry data processing and compound annotation in MZmine 3

Genetic in vivo engineering of human T lymphocytes in mouse models

Digital postprocessing and image segmentation for objective analysis of colorimetric reactions

查看全部

扫码咨询