Standard Operating Procedure: Vibratomes
Hong-Yuan Chu
Abstract
This protocol details the procedure of the standard operating procedure (SOP) describes the safe use of vibratomes in the Chu lab.
Attachments
Steps
Tissue sectioning procedure
Remove the blade from manufacturer package and wash it with ethanol and distilled water.
Install the blade carefully in the vibratome according to the procedure describes in the manual for the specific vibratome used.
Mount the tissue onto chuck and place in vibratome according to the procedure describes in the manual for the specific vibratome used.
Submerge the tissue in media appropriate to sectioning purpose (e.g. ice-cold 1x PBS).
Slice the tissue at desired thickness using the procedure describes in the manual for the specific vibratome used.
Remove the blade carefully after slicing the tissue and dispose of in a sharps-container.
Cleanse the vibratome with deionized water.
