Spot detection on structured background protocol
Bin Fu
Published: 2023-11-29 DOI: 10.17504/protocols.io.36wgq3y23lk5/v1
Abstract
This protocol details the steps for using the spot detection code
Attachments
Steps
1.
Put sub-diffraction beads images in the folder ‘ area_threshold’ and run determineAreaThres.m to find the maximum area in pixel for a diffraction-limited object.
2.
Put negative control images in the folder ‘negative_control’ and run determineRad.m to determined the steepness and integrated gradient used in the study.
3.
Put images in ‘image’ folder and run detection.m for diffraction-limited puncta detection
