Self-made chrome alum gelatin coated slides

Heat up 200mL ddH2O to 50°C on hot plate using a magnetic stirrer.

Add 1g gelatin gradually until completely dissolved to prevent formation of clumps. Solution has to be clear.

Let solution cool down to room temperature and add 0.1g Chrome potassium sulfate until completely dissolved.

Either proceed immediately or store the solution for a few days @4°C . If stored, let the solution warm-up to room temperature

before use.

Pour prepared chrome alum gelatin solution in a staining dish preheated at 60°C.

Incubate to-be-treated slides in 70% (v/v) Ethanol for 0h 10m 0s to clean them.

Rinse in distilled water, dip at least 3 times to remove alcohol.

Dip the slides 5 times in the solution, 0h 0m 10s each.

Leave the slides to drain for a few minutes onto a filter paper.

Dry the treated slides in an oven at 37°C over night.

Store the slides in a dry box and protect from dust.