RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure

Jill M. Brown, Sara De Ornellas, Eva Parisi, Lothar Schermelleh, Veronica J. Buckle

Published: 2022-04-03 DOI: 10.1038/s41596-022-00685-8

super-resolution microscopy

RASER-FISH

3D chromatin structure

non-denaturing FISH

nuclear organization

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DNA fluorescence insitu hybridization (FISH) has been a central technique in advancing our understanding of how chromatin is organized within the nucleus. With the increasing resolution offered by super-resolution microscopy, the optimal maintenance of c
Introduction
Materials
Procedure
Troubleshooting
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Anticipated results
- Reporting Summary
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References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure

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