Quantitative profiling of posttranslational modifications of pathological tau via sarkosyl fractionation and mass spectrometry

Supplementary Information

Supplementary Fig. 1.

Supplementary Table 1

Transition list including monitored precursors and fragments of the FLEXITau assay.

Supplementary Data 1

Sequence of FLEXITau expression vector and the corresponding protein product.

Supplementary Data 2

Skyline library for FLEXITau data evaluation containing all monitored precursors and fragments of the FLEXITau assay.

Supplementary Data 3

Example for evaluating FLEXITau data, including the calculation of absolute total tau amount and the relative modification extent.