Purification and functional characterization of novel human skeletal stem cell lineages

Malachia Y. Hoover, Thomas H. Ambrosi, Holly M. Steininger, Lauren S. Koepke, Yuting Wang, Liming Zhao, Matthew P. Murphy, Alina A. Alam, Elizabeth J. Arouge, M. Gohazrua K. Butler, Eri Takematsu, Suzan P. Stavitsky, Serena Hu, Debashis Sahoo, Rahul Sinha, Maurizio Morri, Norma Neff, Julius Bishop, Michael Gardner, Stuart Goodman, Michael Longaker, Charles K. F. Chan

Published: 2023-06-13 DOI: 10.1038/s41596-023-00836-5

human skeletal stem cells

flow cytometric isolation

functional characterization

skeletally restricted lineages

single-cell RNA sequencing

AI 解读

Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications

High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation

Longitudinal interrogation of sympathetic neural circuits and hemodynamics in preclinical models

Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Combining surface-accessible Ag and Au colloidal nanomaterials with SERS for in situ analysis of molecule–metal interactions in complex solution environments

Generation of mouse–human chimeric embryos

Labeling and tracking of immune cells in ex vivo human skin

Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters

Mutate-and-chemical-shift-fingerprint (MCSF) to characterize excited states in RNA using NMR spectroscopy

Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing

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Human skeletal stem cells (hSSCs) hold tremendous therapeutic potential for developing new clinical strategies to effectively combat congenital and age-related musculoskeletal disorders. Unfortunately, refined methodologies for the proper isolation of bon
Introduction
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ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Purification and functional characterization of novel human skeletal stem cell lineages

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