Protocol 2 Keira Smith Published: 2021-08-24 DOI: 10.17504/protocols.io.bw8aphse AI 解读 浏览 1119 Abstract Text of the description Steps1. 推荐阅读 Nature Protocols Protocols IO Current Protocols Fabrication of angstrom-scale two-dimensional channels for mass transport Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy Profiling native pulmonary basement membrane stiffness using atomic force microscopy Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes 查看全部 In-vitro GCase Activity Assay ASAPCRN GCase GCase activity Isolation of NeuN+ cells from brain tissue (for CUT and RUN) ASAPCRN URMC TriState SenNet Mouse Lung Digestion cellular senescence lung tissue Dissociation Long Amplicon Nanopore Sequencing for Dual-Typing RdRp and VP1 Genes of Norovirus Genogroups I and II in Wastewater Norovirus Oxford Nanopore Technologies Long Amplicon Wastewater Sequencing Assessment of prepulse inhibition (PPI) of the acoustic startle reflex in rodents Histology, Immunohistochemistry and Imaging ASAPCRN Differentiation NPCs to Dopaminergic/Midbrain Neurons differentiation dxn NPC Dopaminergic Midbrain Neurons ASAPCRN LRRK2-RCKW: MLi-2: E11 DARPin cryo-EM sample preparation ASAPCRN Tissue collection Characterizing spatial and temporal properties of ER-phagy receptors ASAPCRN 查看全部 Improving Species Level-taxonomic Assignment from 16S rRNA Sequencing Technologies 16S rRNA gene amplicons DADA2 microbiota composition metagenomics species-classification Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes Arabidopsis thaliana density gradient centrifugation EDTA leaf trichome Generation of Bispecific Antibodies by Functionalized Poly-ADP-Ribose Polymers antibody bispecific antibody chemical conjugation immunotherapy PARP1 poly-ADP-ribose post-translational modification High-Throughput Gene Replacement in Aspergillus fumigatus Aspergillus fumigatus fusion PCR genome-wide knockout library high-throughput gene editing Human Umbilical Cord Mesenchymal Stem Cell–Based in vitro Model for Neurotoxicity Testing cytotoxicity test mesenchymal stem cells neuron-like cells neuronal markers Culture of Rat Primary Cortical Cells for Microelectrode Array (MEA) Recordings to Screen for Acute and Developmental Neurotoxicity acute subchronic and chronic exposure microelectrode array (MEA) recordings neuronal activity analysis neurotoxicity screening rat primary cortical cultures Hi-C 3.0: Improved Protocol for Genome-Wide Chromosome Conformation Capture chromatin interactions chromosome conformation capture cross-linking Hi-C restriction enzyme Fluorescent Protein Production, Purification, and Coupling to Microspheres fluorescent proteins full-spectrum cytometry microspheres protein expression protein purification ntEdit+Sealer: Efficient Targeted Error Resolution and Automated Finishing of Long-Read Genome Assemblies assembly finishing Bloom filter hybrid assembly polishing k-mer long-read genome assembly Chloroplast Transformation in Arabidopsis Arabidopsis biolistic transformation chloroplast transformation plastid transformation transplastomic plant 查看全部
