Protocol 1 Keira Smith Published: 2021-08-24 DOI: 10.17504/protocols.io.bwgfpbtn mykeyword1 mykeyword2 AI 解读 浏览 1197 Abstract Main Steps1. Step 1 推荐阅读 Nature Protocols Protocols IO Current Protocols Transplantation of intestinal organoids into a mouse model of colitis Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy Design and fabrication of wearable electronic textiles using twisted fiber-based threads TAC–TIC, a high-throughput genetics method to identify triggers or blockers of bacterial toxin–antitoxin systems Microbiology techniques Phage biology Prokaryote Bacteriophages Bacterial genetics Kit-based synthesis of 2-deoxy-2-[18F]-fluoro-d-sorbitol for bacterial imaging Single-cell RNA-seq of maize meiocytes and pollen grains Precise mutagenesis in zebrafish using cytosine base editors A general protocol for precise syntheses of ordered mesoporous intermetallic nanoparticles Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome Multiplexed single-cell analysis of organoid signaling networks 查看全部 In vitro GCase activity assay (total cell lysate) In vitro GCase activity assay total cell lysate ASAPCRN Seahorse protocol for islets using Xfe24 Analyzer Microsomal membrane isolation from cell culture ASAPCRN Static Incubation of Pancreatic Islets RT-QuIC alpha-synuclein ASAPCRN Adult mouse kidney dissociation (on ice) kidney dissociation single cell Efficacy and safety of statin therapy in kidney transplant recipients: a meta-analysis CD8 Cell Density In Substantia Nigra And Cerebral Peduncle Image Analysis ASAPCRN Human Brain Substantia Nigra Cerebral Peduncle CD8 Parkinson’s disease QuPath NZConnect Image Analysis Pathology Protocol Normative Gait Data ZHAW Movement Laboratory gait walking normative data three-dimensional A fast, easy, cost-free method to remove excess dye or drug from exosome solution 查看全部 Expanding the Perseus Software for Omics Data Analysis With Custom Plugins MaxQuant omics data analysis Perseus plugin development quantitative proteomics Differentiation, Transcriptomic Profiling, and Calcium Imaging of Human Hypothalamic Neurons differentiation human hypothalamus neuron obesity pluripotent stem cell POMC protocol Genome Editing with CRISPR-Cas: An Overview Cas9 Cas12a CRISPR gene editing genome editing Massively Parallel Sequencing of the Mitogenome from Human Hair Shafts in Forensic Investigations DNA extraction of human hair forensic genetics mitochondrial genome MPS multiplex PCR Modified Cross-Linking, Ligation, and Sequencing of Hybrids (qCLASH) to Identify MicroRNA Targets Argonaute ligation MicroRNA qCLASH ribonomics Nanopore-Enabled Microbiome Analysis: Investigating Environmental and Host-Associated Samples in Rainbow Trout Aquaculture 16S rRNA gene aquaculture eDNA fish microbiome nanopore sequencing Phenotypic Characterization and Isolation of Myeloid-Derived Suppressor Cells chronic inflammation flow cytometry immunofluorescence imaging immunosuppression inflammatory mouse model myeloid-derived suppressor cells MDSC patient MDSC Evaluating the Clinical and Immune Responses to Spotted Fever Rickettsioses in the Guinea Pig-Tick-Rickettsia System animal model Cavia porcellus rickettsia rickettsiosis tick transmission Human iPSC-Derived Blood-Brain Barrier Models: Valuable Tools for Preclinical Drug Discovery and Development? blood-brain barrier (BBB) in vitro model CNS disease drug permeability screening human-induced pluripotent stem cells (hiPSC) preclinical drug discovery Production and Thermal Exchange of Conditional Peptide-MHC I Multimers CD8+ T cell staining MHC class I multimer peptide thermal exchange 查看全部