Protocol 1 Keira Smith Published: 2021-08-24 DOI: 10.17504/protocols.io.bwgfpbtn mykeyword1 mykeyword2 AI 解读 浏览 1104 Abstract Main Steps1. Step 1 推荐阅读 Nature Protocols Protocols IO Current Protocols Fabrication of angstrom-scale two-dimensional channels for mass transport Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy Profiling native pulmonary basement membrane stiffness using atomic force microscopy Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes 查看全部 In-vitro GCase Activity Assay ASAPCRN GCase GCase activity Isolation of NeuN+ cells from brain tissue (for CUT and RUN) ASAPCRN URMC TriState SenNet Mouse Lung Digestion cellular senescence lung tissue Dissociation Long Amplicon Nanopore Sequencing for Dual-Typing RdRp and VP1 Genes of Norovirus Genogroups I and II in Wastewater Norovirus Oxford Nanopore Technologies Long Amplicon Wastewater Sequencing Assessment of prepulse inhibition (PPI) of the acoustic startle reflex in rodents Histology, Immunohistochemistry and Imaging ASAPCRN Differentiation NPCs to Dopaminergic/Midbrain Neurons differentiation dxn NPC Dopaminergic Midbrain Neurons ASAPCRN LRRK2-RCKW: MLi-2: E11 DARPin cryo-EM sample preparation ASAPCRN Tissue collection Characterizing spatial and temporal properties of ER-phagy receptors ASAPCRN 查看全部 Generation of 3D Tumor Spheroids with Encapsulating Basement Membranes for Invasion Studies spheroid basement membrane invasion 3D-model Optimizing PCR for Mouse Genotyping: Recommendations for Reliable, Rapid, Cost Effective, Robust and Adaptable to High-Throughput Genotyping Protocol for Any Type of Mutation 3Rs replacement reduction refinement automation genotyping molecular biology mouse model PCR reproducibility Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host-Microbe Interactions fungal genomics maize plant genomics plant-microbe interactions unique molecular identifiers Ustilago maydis virulence factors ezTrack—A Step-by-Step Guide to Behavior Tracking behavioral analysis fear conditioning freeze position tracking Assessing Protein Synthesis and Degradation Rates in Arabidopsis thaliana Using Amino Acid Analysis 13CO2 labeling protein degradation protein synthesis Hepatitis E Virus: Isolation, Propagation, and Quantification HEV isolation propagation virus quantification Murine Norovirus: Additional Protocols for Basic and Antiviral Studies baculovirus expression circular polymerase extension reaction murine norovirus neonatal infections tuft cell-enriched murine intestinal enteroid culture Analysis of Genetic Ancestry from NGS Data Using EthSEQ ancestry analysis NGS SNPs Scaled Isolation of Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles EVs exosomes large-scale preparation MSCs Genomic Epidemiology Analysis of Infectious Disease Outbreaks Using TransPhylo genomic epidemiology infectious disease outbreak phylogenetics transmission analysis 查看全部
