Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions

Anais Sahabian, Julia Dahlmann, Ulrich Martin, Ruth Olmer

Published: 2021-02-11 DOI: 10.1038/s41596-020-00470-5

human pluripotent stem cells

definitive endoderm

cryopreservation

scalable culture

endodermal lineages

AI 解读

Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq

Generation of heart-forming organoids from human pluripotent stem cells

Design and fabrication of wearable electronic textiles using twisted fiber-based threads

Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo

Chromatin accessibility profiling by ATAC-seq

Deoxyfluorination of phenols for chemoselective18F-labeling of peptides

Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction

Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening

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The endodermal germ layer gives rise to respiratory epithelium, hepatocytes, pancreatic cells and intestinal lineages, among other cell types. These lineages can be differentiated from human pluripotent stem cells (hPSCs) via a common definitive endoderm
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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ACKNOWLEDGMENTS

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Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions

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