Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions

Anais Sahabian, Julia Dahlmann, Ulrich Martin, Ruth Olmer

Published: 2021-02-11 DOI: 10.1038/s41596-020-00470-5

human pluripotent stem cells

definitive endoderm

cryopreservation

scalable culture

endodermal lineages

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Supplementary information

Supplementary Data

Source data for cell viability results are included in the ‘Anticipated Results’ section. Cell viability was assessed by Beckman Coulter Vi-CELL X.

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions

Supplementary information

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