Primer Design for Restriction Enzyme Cloning (E6901)

New England Biolabs

Published: 2022-02-22 DOI: 10.17504/protocols.io.bhk3j4yn

Restriction endonuclease cloning

cloning into pTXB1

restriction site

Primer desing for cloning

cloning with restriction enzymes

forward primer reverse primer

N-terminal fusion

C-terminal fusion

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Abstract

Guidelines for Primer Design for Restriction Enzyme Cloning (E6901).

Before start

Steps

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