Preparation of BG-11 media Agar

Ashwinuday

Published: 2021-10-18 DOI: 10.17504/protocols.io.by6rpzd6

Abstract

This is for making solid media of BG-11

Steps

1.

To make BG-11 Agar plates, add Agar-Agar Type-1 equivalent to 1.5% (w/v) of liquid media and autoclave.

2.

Then before it solidifies (when the flask is bearable to touch), add the required antibiotics to the media.

3.

Pour ~30-40 ml into plates soon after.

4.

Keep the plates opened in the hood.

5.

Once it solidifies in the plates then close those and keep the plates inverted into a cold storage.

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