Precise genome engineering inPseudomonasusing phage-encoded homologous recombination and the Cascade–Cas3 system

Xue Wang, Wentao Zheng, Yandong Xia, Shiqing Gao, Diao Zhou, Vinothkannan Ravichandran, Chanjuan Jiang, Qiang Tu, Yulong Yin, Youming Zhang, Jun Fu, Ruijuan Li, Jia Yin

Published: 2023-08-24 DOI: 10.1038/s41596-023-00856-1

Pseudomonas genome engineering

Phage-encoded homologous recombination

Cascade-Cas3 system

Recombineering efficiency

Genetic manipulation

AI 解读

Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications

High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation

Longitudinal interrogation of sympathetic neural circuits and hemodynamics in preclinical models

Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Combining surface-accessible Ag and Au colloidal nanomaterials with SERS for in situ analysis of molecule–metal interactions in complex solution environments

Generation of mouse–human chimeric embryos

Labeling and tracking of immune cells in ex vivo human skin

Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters

Mutate-and-chemical-shift-fingerprint (MCSF) to characterize excited states in RNA using NMR spectroscopy

Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing

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Sections

Figures

References

Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Determination of the resistance and transformation characteristics of Pseudomonas
- Two-step seamless modification and three-step seamless modification
Acknowledgements

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Precise genome engineering inPseudomonasusing phage-encoded homologous recombination and the Cascade–Cas3 system

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