PLOS ONE Anna Guzek Published: 2022-08-20 DOI: 10.17504/protocols.io.b5w6q7he AI 解读 浏览 1164 Abstract Analysis of auditory deficits of 1,012 children aged 6 to 9 confirmed the adequacy of use ofthe APD testing in 6-year-old children Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters Constructing and interpreting volcano plots and activity maps to navigate homogeneous catalyst landscapes The use of methanol as a C1 building block Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases The maternal serum metabolome by multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform and standardized data workflow for large-scale epidemiological studies 3D human induced pluripotent stem cell–derived bioengineered skeletal muscles for tissue, disease and therapy modeling Temporally resolved transcriptional recording inE. coliDNA using a Retro-Cascorder Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing 查看全部 SOP for Immune cells isolation mouse adult brain with CD45 beads ASAPCRN Test for admin publication GRAB sensor imaging in mouse striatal slices GRAB sensors imaging mouse ex vivo slices ASAPCRN Cost-effective Approach for Assessing Chemosensory Response in Parhyale hawaiensis: A Time-to-Event Experiment Chemosensation Feeding behaviour Parhyale hawaiensis Y-Maze Time-to-event Kaplan-Meier OR Cox regression survival analysis A Systematic Comparison of Protocols for Recovery of High-Quality RNA from Human Islets Extracted by Laser Capture Microdissection laser capture microdissection RNA extraction human islets Qubit4 Fluorometer Protocol: Invitrogen dsDNA HS Assay Kit (REF 32854) Mitochondrial isolation protocol Mitochondrial isolation HeLa cells ASAPCRN Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765) RCA-NGS for RNA viruses Oxford Nanopore Technology RNA virus Sequence method MinION Nanopore sequencing RCA-NGS DNA Extraction from 0.22µm Sterivex Filters - Qiagen Blood and Tissue with Inhibitor Removal 查看全部 Acute Myeloid Leukemia Minimal Residual Disease Detection: The Difference from Normal Approach acute myeloid leukemia AML flow cytometry minimal residual disease MRD ezTrack—A Step-by-Step Guide to Behavior Tracking behavioral analysis fear conditioning freeze position tracking Ultrasound-Guided Approaches to Improve Orthotopic Mouse Xenograft Models for Hepatocellular Carcinoma doxorubicin echography echo-guided injection hepatocarcinoma Huh-7 orthotopic Visualization of the Skeletal Muscle Stem Cell Niche in Fiber Bundles microenvironment MuSCs satellite cells skeletal muscle stem cell niche quiescence Triplet-Primed PCR Assays for Accurate Screening of FMR1 CGG Repeat Expansion and Genotype Verification capillary electrophoresis FMR1 fragile X syndrome melting curve analysis screening triplet-primed PCR Isolation of Adult Mouse Cardiac Fibroblasts cardiac fibroblasts differentiation fibrosis flow cytometry heart failure single-cell Using Fluorescent GAP Indicators to Monitor ER Ca2+ aequorin Ca2+ calibration fluorescence GFP imaging Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes Arabidopsis thaliana density gradient centrifugation EDTA leaf trichome An Updated Guide to Robust Statistical Methods in Neuroscience curvature heteroscedasticity non-normality outliers skewed distributions STARR-seq and UMI-STARR-seq: Assessing Enhancer Activities for Genome-Wide-, High-, and Low-Complexity Candidate Libraries cis-regulatory element enhancer functional genomics gene expression gene regulation massively parallel reporter assay MPRA STARR-seq transcriptional regulation UMI-STARR-seq 查看全部