Overall protocol for top-down LC-MS/MS of human spleen tissue
Jeannie Camarillo, Bryon Drown, Neil Kelleher
Published: 2022-03-02 DOI: 10.17504/protocols.io.b5tgq6jw
Abstract
Overview description of the process for acquiring top-down LC-MS/MS data on human spleen tissue
Steps
Tissue Collection
1.
Spleen tissue was provided by University of Florida TMC. Tissue was collected and prepared according to the following:
Sample Preparation
2.
Perform LC based Proteomics on adjacent tissue sections: