Overall protocol for top-down LC-MS/MS of human heart tissue
Jeannie Camarillo, Bryon Drown, Neil Kelleher
Published: 2022-03-02 DOI: 10.17504/protocols.io.b5tjq6kn
Abstract
Overview description of the process for acquiring top-down LC-MS/MS data on human heart tissue
Steps
Tissue Collection
1.
Heart tissue was provided by University of Washington. Tissue was collected and prepared according to the following:
Protocol for Tissue Collection from Organ Procurement Organization
Sample Preparation
2.
Perform LC based Proteomics on adjacent tissue sections:
