Nissl (Cresyl Violet) staining
Christiana.bjorkli, Karoline Hovde, Bruno Monterotti
Published: 2022-03-19 DOI: 10.17504/protocols.io.dm6gpbzxplzp/v1
Abstract
Nissl (Cresyl Violet) staining protocol
Steps
1.
Dehydrate sections – 10 dips in each: 50, 70, 80, 90, 100, 100, and 100% ethanol
2.
Leave in xylene for 2min
3.
Rehydrate sections – 10 dips in each: 100, 100, 100, 90, 80, 70, and 50% ethanol
4.
Quick wash in running water
5.
Leave sections in Cresyl Violet for 3min on shake
6.
Leave sections in Citric Acid for 1min
6.1.
Dip in running water 3x
6.2.
Dip in Citric Acid 3x
6.3.
Dip in running water 3x
7.
Leave sections in Cresyl Violet for 2min on shaker
8.
Repeat step 6
9.
Dehydrate sections – 10 dips in each: 50, 70, 80, 90, 100, 100, and 100% ethanol
10.
Leave in Xylene for at least 5min
11.
Coverslip with Xylene + Entellan
