Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

Anne K. Engmann, John J. Hatch, Prakruti Nanda, Priya Veeraraghavan, Abdulkadir Ozkan, Alexandros Poulopoulos, Alexander J. Murphy, Jeffrey D. Macklis

Published: 2022-01-11 DOI: 10.1038/s41596-021-00638-7

Neuronal subtype

Growth cone

Fluorescent sorting

Subcellular analysis

Proteomic investigation

AI 解读

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During neuronal development, growth cones (GCs) of projection neurons navigate complex extracellular environments to reach distant targets, thereby generating extraordinarily complex circuitry. These dynamic structures located at the tips of axonal projec
Introduction
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Anticipated results
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ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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