Multigenerational laboratory culture of pelagic ctenophores and CRISPR–Cas9 genome editing in the lobateMnemiopsis leidyi

J. S. Presnell, M. Bubel, T. Knowles, W. Patry, W. E. Browne

Published: 2022-06-12 DOI: 10.1038/s41596-022-00702-w

Supplementary information

Demonstration of target feeding a Mnemiopsis leidyi adult. Zebrafish larvae ( Danio rerio ) that have been pre-washed with ASW are delivered directly to the tentacle lined feeding grooves via plastic pipette where they become ensnared in adhesive material produced by colloblast cells lining the tentilla. The trapped fish larvae are then transported orally, and engulfed

Supplementary Video 2

Juvenile Mnemiopsis leidyi cydippid capturing prey in tentacles. During juvenile stages, the two main tentacles extend into the surrounding water column from which side branching tentilla are then deployed to form a dense network of sticky colloblast cells used to ensnare nearby plankton

Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo

Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening

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Multigenerational laboratory culture of pelagic ctenophores and CRISPR–Cas9 genome editing in the lobateMnemiopsis leidyi

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