Luminescent Conjugated Oligothiophenes (LCO) Staining

Generate tissue sections using standard microtome sectioning protocols.

Heat tissue dry tissue sections for 1h 0m 0s at 60°C

Safety information

Please refer to the Xylene Data Safety Sheet and follow local approved risk assessements and protocols for safe handling and disposal of xylene.

De-paraffinise sections by 0h 5m 0s washes in xylene (x2), 0h 5m 0s 100 % ethanol (x2) and 0h 5m 0s in DEPC H₂O (x1).

Circle tissue on slide using a hydrophobic ImmEdge^TM PAP pen (Vector laboratories) to contain

solution.

Prepare 1:500 pFTAA solution (diluted in PBS). All steps from here should be performed away from light.

Pipette pFTAA solution onto tissue and incubate at room temperature in a humidified chamber for 0h 30m 0smins.

Wash slides in PBS 3x.

Coverslip slides using Hydromount^TM aqueous non-fluorescing mounting media (National Diagnostics).