LB Broth

sgoodwin

Published: 2023-06-22 DOI: 10.17504/protocols.io.yxmvm375nl3p/v1
AI 解读
浏览 1220

Abstract

Process for producing LB broth growth medium

Steps

Making the LB broth

1.

Dilute 20g of LB powder in 800mL distilled water

2.

Stir until fully dissolved with magnetic stirrer

3.

Transfer to a Duran flask, and sterilise by autoclaving

推荐阅读

Nature Protocols
Protocols IO
Current Protocols

Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing

Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters

Constructing and interpreting volcano plots and activity maps to navigate homogeneous catalyst landscapes

The use of methanol as a C1 building block

Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases

The maternal serum metabolome by multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform and standardized data workflow for large-scale epidemiological studies

3D human induced pluripotent stem cell–derived bioengineered skeletal muscles for tissue, disease and therapy modeling

Temporally resolved transcriptional recording inE. coliDNA using a Retro-Cascorder

Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging

Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing

查看全部

扫码咨询