LB Broth

sgoodwin

Published: 2023-06-22 DOI: 10.17504/protocols.io.yxmvm375nl3p/v1
AI 解读
浏览 1151

Abstract

Process for producing LB broth growth medium

Steps

Making the LB broth

1.

Dilute 20g of LB powder in 800mL distilled water

2.

Stir until fully dissolved with magnetic stirrer

3.

Transfer to a Duran flask, and sterilise by autoclaving

推荐阅读

Nature Protocols
Protocols IO
Current Protocols

Opto-juxtacellular interrogation of neural circuits in freely moving mice

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Self-assembled ultraflexible probes for long-term neural recordings and neuromodulation

Acute head-fixed recordings in awake mice with multiple Neuropixels probes

Creating hierarchical pores in metal–organic frameworks via postsynthetic reactions

SorTn-seq: a high-throughput functional genomics approach to discovering regulators of bacterial gene expression

Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages

FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing

Scalable tissue labeling and clearing of intact human organs

Analyzing somatic mutations by single-cell whole-genome sequencing

查看全部

扫码咨询