KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal–assisted labeling

Chuan He, Tong Wu, Ruitu Lyu, Allen C. Zhu, Diana C. West-Szymanski, Xiaocheng Weng, Mengjie Chen

Published: 2022-01-09 DOI: 10.1038/s41596-021-00647-6

KAS-seq

N3-kethoxal

single-stranded DNA

transcription dynamics

genome-wide sequencing

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Transcription and its dynamics are crucial for gene expression regulation. However, very few methods can directly read out transcriptional activity with low-input material and high temporal resolution. This protocol describes KAS-seq, a robust and sensiti
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal–assisted labeling

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