Isolation of mouse pancreatic islet Procr+progenitors and long-term expansion of islet organoids in vitro

Jingqiang Wang, Daisong Wang, Xinyi Chen, Shubo Yuan, Lanyue Bai, Chunye Liu, Yi Arial Zeng

Published: 2022-04-07 DOI: 10.1038/s41596-022-00683-w

Procr+ progenitors

islet organoids

in vitro expansion

beta cell generation

glucose homeostasis

AI 解读

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

查看全部

Sections

Figures

References

Insulin production is required for glucose homeostasis. Pancreatic islet β cells are the only cells that produce insulin in humans; however, generation of functional β cells invitro from embryonic or adult tissues has been challenging. He
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- References
References
ACKNOWLEDGMENTS

Zhou, Q. & Melton, D. A. Pancreas regeneration. Nature 557, 351–358 (2018).
Greggio, C. et al. Artificial three-dimensional niches deconstruct pancreas development in vitro. Development 140, 4452–4462 (2013).
Sugiyama, T. et al. Reconstituting pancreas development from purified progenitor cells reveals genes essential for islet differentiation. Proc. Natl Acad. Sci. USA 110, 12691–12696 (2013).
Ramiya, V. K. et al. Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cells. Nat. Med. 6, 278–282 (2000).
Jin, L. et al. Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel. Proc. Natl Acad. Sci. USA 110, 3907–3912 (2013).
Bonner-Weir, S. et al. In vitro cultivation of human islets from expanded ductal tissue. Proc. Natl Acad. Sci. USA 97, 7999–8004 (2000).
Gao, R., Ustinov, J., Korsgren, O. & Otonkoski, T. In vitro neogenesis of human islets reflects the plasticity of differentiated human pancreatic cells. Diabetologia 48, 2296–2304 (2005).
Yatoh, S. et al. Differentiation of affinity-purified human pancreatic duct cells to beta-cells. Diabetes 56, 1802–1809 (2007).
Rovira, M. et al. Isolation and characterization of centroacinar/terminal ductal progenitor cells in adult mouse pancreas. Proc. Natl Acad. Sci. USA 107, 75–80 (2010).
Huch, M. et al. Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis. EMBO J. 32, 2708–2721 (2013).
Minami, K. et al. Lineage tracing and characterization of insulin-secreting cells generated from adult pancreatic acinar cells. Proc. Natl Acad. Sci. USA 102, 15116–15121 (2005).
Seaberg, R. M. et al. Clonal identification of multipotent precursors from adult mouse pancreas that generate neural and pancreatic lineages. Nat. Biotechnol. 22, 1115–1124 (2004).
Smukler, SimonR. et al. The adult mouse and human pancreas contain rare multipotent stem cells that express insulin. Cell Stem Cell 8, 281–293 (2011).
Hogrebe, N. J., Augsornworawat, P., Maxwell, K. G., Velazco-Cruz, L. & Millman, J. R. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells. Nat. Biotechnol. 38, 460–470 (2020).
Pagliuca, FeliciaW. et al. Generation of functional human pancreatic β cells in vitro. Cell 159, 428–439 (2014).
Rezania, A. et al. Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells. Nat. Biotechnol. 32, 1121–1133 (2014).
Yoshihara, E. et al. Immune-evasive human islet-like organoids ameliorate diabetes. Nature 586, 606–611 (2020).
González, B. J., Creusot, R. J., Sykes, M. & Egli, D. How safe are universal pluripotent stem cells? Cell Stem Cell 26, 307–308 (2020).
Valdez, I. A., Teo, A. K. K. & Kulkarn, R. N. Cellular stress drives pancreatic plasticity. Sci. Transl. Med. 7, 273ps272 (2015).
Wang, D. et al. Long-term expansion of pancreatic islet organoids from resident Procr+ progenitors. Cell 180, 1198–1211.e19 (2020).
Gittes, G. K. Developmental biology of the pancreas: a comprehensive review. Dev. Biol. 326, 4–35 (2009).
Lammert, E., Cleaver, O. & Melton, D. Induction of pancreatic differentiation by signals from blood vessels. Science 294, 564–567 (2001).
Zhou, Q., Brown, J., Kanarek, A., Rajagopal, J. & Melton, D. A. In vivo reprogramming of adult pancreatic exocrine cells to β-cells. Nature 455, 627–632 (2008).
Lemper, M. et al. Reprogramming of human pancreatic exocrine cells to β-like cells. Cell Death Differ. 22, 1117–1130 (2014).
Lee, J. et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. eLife 2, e00940 (2013).
Pan, F. C. & Wright, C. Pancreas organogenesis: from bud to plexus to gland. Dev. Dyn. 240, 530–565 (2011).
Furuyama, K. et al. Diabetes relief in mice by glucose-sensing insulin-secreting human α-cells. Nature 567, 43–48 (2019).
Clevers, H. Modeling development and disease with organoids. Cell 165, 1586–1597 (2016).
McCall, M. D., Maciver, A. H., Pawlick, R. L., Edgar, R. & Shapiro, A. M. J. Histopaque provides optimal mouse islet purification kinetics: comparison study with Ficoll, iodixanol and dextran. Islets 3, 144–149 (2014).
Zmuda, E. J., Powell, C. A. & Hai, T. A method for murine islet isolation and subcapsular kidney transplantation. J. Vis. Exp. 2011, 2096 (2011).
Akira Shiroi, M. et al. Identification of insulin-producing cells derived from embryonic stem cells by zinc-chelating dithizone. Stem Cells Int. 20, 284–292 (2002).
Crowley, L. C., Marfell, B. J., Christensen, M. E., and Waterhouse, N. J. Measuring cell death by trypan blue uptake and light microscopy. Cold Spring Harb. Protoc. https://doi.org/10.1101/pdb.prot087155 (2016).
Corbin, K. L. et al. A practical guide to rodent islet isolation and assessment revisited. Biol. Proced. Online 23, 7 (2021).
Huang, C. & Gu, G. Effective isolation of functional islets from neonatal mouse pancreas. J. Vis. Exp. 2017, 55160 (2017).
Junod, A., Lambert, A. E., Stauffacher, W. & Renold, A. E. Diabetogenic action of streptozotocin: relationship of dose to metabolic response. J. Clin. Invest. 48, 2129–2139 (1969).
Lenzen, S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia 51, 216–226 (2007).
Deeds, M. C. et al. Single dose streptozotocin-induced diabetes: considerations for study design in islet transplantation models. Lab. Anim. 45, 131–140 (2011).

Isolation of mouse pancreatic islet Procr+progenitors and long-term expansion of islet organoids in vitro

推荐阅读