Human urine procurement and processing
hannah.anvari, asma.giornazi, shriya.shah, maryellen.pavone, francesca.e.duncan duncan
Abstract
Purpose: This protocol is intended for use in the collection and storage of human urine in a research setting. The protocol details the collection, processing, and long-term storage for downstream analysis alongside other tissues or samples of interest (i.e. follicular fluid, ovary).
Steps
A urine sample is collected in a standard specimen cup during pre-op from a nurse. Once the urine sample has been collected it is placed immediately on ice following collection. A research coordinator will bring the urine sample to the lab on ice (2-4 °C). Urine samples should be transported in a blue, biohazard labeled cooler bag (Fig. 1A, B).
On arrival the urine sample biospecimen cup is photographed and then aliquoted from the specimen cup (Fig. 1C) into 5 mL cryovials (Fig. 1D) and then stored at -80°C until analysis.
