High-throughput proteomic sample preparation using pressure cycling technology

Wei Liu, Chen Chen, Xue Cai, Zhangzhi Xue, Chunlong Wu, Rui Sun, Liujia Qian, Liang Yue, Weigang Ge, Xiao Yi, Huanhuan Gao, Jing Yu, Luang Xu, Yi Zhu, Tiannan Guo

Published: 2022-08-04 DOI: 10.1038/s41596-022-00727-1

mass spectrometry

pressure cycling technology

proteomic sample preparation

clinical proteomics

tissue lysis

AI 解读

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High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling technology (PCT)-assisted sample preparation for proteomic analysis of
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- References
References
ACKNOWLEDGMENTS

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High-throughput proteomic sample preparation using pressure cycling technology

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