Gel Electrophoresis
NUS iGEM
Abstract
2023 NUS-Singapore iGEM team followed this protocol for gel electrophoresis to isolate the DNA fragments from the PCR products.
Steps
Set up the required apparatus:
Secure the gel tray tightly onto the gel caster.
Place an 8-well or 15-well comb into the gel tray.
Make an agarose gel by following the steps in the "Preparation of Agarose Gel" protocol. Usually, a 1% agarose gel is used.
After the agarose gel has solidified in the gel tray, remove the gel tray from the gel caster and place it in a buffer tank with pre-filled 1x TAE buffer.
Add the correct DNA ladder and the sample(s) into the wells.
Close the buffer tank with the tank lid, ensuring that the colour of the electrodes on the tank lid is the same as the one on the buffer tank.
Run the gel electrophoresis at 110V for 40 minutes, making sure that bubbling occurs when the gel electrophoresis begins.
Upon finishing gel electrophoresis, put the agarose gel onto a UV Sample Tray, next, put it into the Gel Doc EZ System, and then run the gel imaging program on the desktop.
Save the gel image.
