GEARBOCS protocols Justin T Savage, Luke Bradley, Nicholas Brose Published: 2024-08-19 DOI: 10.17504/protocols.io.36wgqnq15gk5/v2 ASAPCRN AI 解读 浏览 2354 Abstract Protocols for AAV production and use of the GEARBOCS system. Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Ligand functionalization of titanium nanopattern enables the analysis of cell–ligand interactions by super-resolution microscopy A microfluidic Braille valve platform for on-demand production, combinatorial screening and sorting of chemically distinct droplets Capturing acyl–enzyme intermediates with genetically encoded 2,3-diaminopropionic acid for hydrolase substrate identification I-TASSER-MTD: a deep-learning-based platform for multi-domain protein structure and function prediction Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia A distal lung organoid model to study interstitial lung disease, viral infection and human lung development Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks Noninvasive in vivo microscopy of single neutrophils in the mouse brain via NIR-II fluorescent nanomaterials Electrochemical molecular intercalation and exfoliation of solution-processable two-dimensional crystals 查看全部 Electrophysiological recording from Brain Slices Protocol ASAPCRN iSeq Bacterial WGS Protocol using a quarter of the reagents iSeq Salmonella Nextera Flex Illumina DNA Prep WGS Vet-LIRN bacterial WGS low-throughput WGS WGS training Intraperitoneal Injection in an Adult Mouse AF0085 IP mice In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Formalin-Fixed Paraffin-Embedded Tissue Protocol for "Neuromelanin accumulation drives endogenous synuclienopathy in non-human primates" Tyrosinase Alpha-synuclein Lewy bodies Marinesco bodies Prion-like spread L-Type Ca2+ Current Protocol Synthetic COI spike-ins for use in metabarcoding-based insect biodiversity surveys Spike-in COI metabarcoding synthetic DNA normalisation calibration Elution, SDS-PAGE, and RNA Purification RNA degradation Protein–RNA interaction RNA-binding sites UV cross-linking Yeast Exosome RNA processing Protocols from: Evolutionary analyses of visual opsin genes in frogs and toads: diversity, duplication, and positive selection Characterization of osmoregulated periplasmic glucans with high resolution LCMS 查看全部 Optimized Workflow for Whole Genome and Transcriptome Next-Generation Sequencing of Single Cells or Limited Nucleic Acid Samples genomics MDA transcriptomics viral nucleic acid next-generation sequencing single cell Antibody-Based CAR T Cells Produced by Lentiviral Transduction CAR T cells chimeric antigen receptor lentiviral transduction Mammalian Reoviruses: Propagation, Quantification, and Storage cesium chloride ultracentrifugation double-stranded RNA mouse cell culture non-enveloped virus plaque assay RNA virus Measurement of Trogocytosis: Quantitative Analyses Validated with Rigorous Controls antibody-drug conjugates complement immune complex clearance immune adherence trogocytosis Validation of a Novel, Flash-Freezing Method: Aluminum Platform DNA fresh tissue immunofluorescence liver pancreas RNA A Patient-to-Model-to-Patient (PMP) Cancer Drug Discovery Protocol for Identifying and Validating Therapeutic Agents Targeting Tumor Regulatory Architecture biomarkers cancer drug discovery regulatory networks systems pharmacology Using Nanocompore to Identify RNA Modifications from Direct RNA Nanopore Sequencing Data bioinformatics direct RNA nanopore sequencing Nanocompore nanopore sequencing RNA RNA modifications Improving Species Level-taxonomic Assignment from 16S rRNA Sequencing Technologies 16S rRNA gene amplicons DADA2 microbiota composition metagenomics species-classification Detection and Quantitation of DNA Damage on a Genome-wide Scale Using RADAR-seq base excision repair DNA damage DNA nick DNA repair next-generation sequencing SMRT sequencing Human Umbilical Cord Mesenchymal Stem Cell–Based in vitro Model for Neurotoxicity Testing cytotoxicity test mesenchymal stem cells neuron-like cells neuronal markers 查看全部
