Fluorescence microscope (Zeiss)

Turn on the white light intensity and shutter

Turn on the zoom controller

Turn on the LED, if using fluorescent mode

Turn on the software on the computer: Pylon viewer

Place sample in the microscope and adjust the eyepiece

Focus the sample using the wheel on the left-hand side whilst observing through the eyepiece

Using the white light shutter on the right to block out the white light, only fluorescence from the sample reaches the eyepiece.

To capture an image, switch to computer mode using the switch on the upper right-hand side.

Press the camera symbol in the software to capture an image. Parameters such as acquisition time can be set in the software.

The best results are obtained when the plastic cover is on and the background light in the room is kept at a minimum.

Turn off the white light, LED, and zoom controller when finished using the microscope.

Enclose with plastic cover.

The software does not connect to the camera:

Make sure the USB cable is plugged in to a USB 3.0 port on the computer. Check that the camera is on. Make sure the correct driver is installed.

The zoom controller, white light or LED don't turn on:

Make sure that the microscope is plugged in.

Nothing can be seen through the eyepiece:

Make sure nothing is blocking the path between the sample and the objective lens. Make sure that the microscope is not switched to camera view.