Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Max Buhlan, Dmitrij Ljaschenko, Nicole Scholz, Tobias Langenhan

Published: 2023-11-09 DOI: 10.1038/s41596-023-00907-7

fluorescence imaging

mechanosensation

Drosophila melanogaster

proprioceptive neurons

force stimulation

AI 解读

Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications

High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation

Longitudinal interrogation of sympathetic neural circuits and hemodynamics in preclinical models

Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Combining surface-accessible Ag and Au colloidal nanomaterials with SERS for in situ analysis of molecule–metal interactions in complex solution environments

Generation of mouse–human chimeric embryos

Labeling and tracking of immune cells in ex vivo human skin

Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters

Mutate-and-chemical-shift-fingerprint (MCSF) to characterize excited states in RNA using NMR spectroscopy

Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing

查看全部

Sections

Figures

References

Mammoto, A., Mammoto, T. & Ingber, D. E. Mechanosensitive mechanisms in transcriptional regulation. J. Cell Sci. 125, 3061–3073 (2012).
Hoffman, B. D., Grashoff, C. & Schwartz, M. A. Dynamic molecular processes mediate cellular mechanotransduction. Nature 475, 316–323 (2011).
Hudspeth, A. J. Integrating the active process of hair cells with cochlear function. Nat. Rev. Neurosci. 15, 600–614 (2014).
Ranade, S. S., Syeda, R. & Patapoutian, A. Mechanically activated ion channels. Neuron 87, 1162–1179 (2015).
Langenhan, T., Piao, X. & Monk, K. R. Adhesion G protein-coupled receptors in nervous system development and disease. Nat. Rev. Neurosci. 17, 550–561 (2016).
Scholz, N., Monk, K. R., Kittel, R. J. & Langenhan, T. Adhesion GPCRs as a putative class of metabotropic mechanosensors. Handb. Exp. Pharmacol. 234, 221–247 (2016).
Wolfenson, H., Yang, B. & Sheetz, M. P. Steps in mechanotransduction pathways that control cell morphology. Annu. Rev. Physiol. 81, 1–21 (2017).
Scholz, N. et al. Molecular sensing of mechano- and ligand-dependent adhesion GPCR dissociation. Nature 615, 945–953 (2023).
Scholz, N. et al. The adhesion GPCR Latrophilin/CIRL shapes mechanosensation. Cell Rep. 11, 866–874 (2015).
Dannhäuser, S. et al. Antinociceptive modulation by the adhesion GPCR CIRL promotes mechanosensory signal discrimination. Elife 9, e56738 (2020).
Scholz, N. et al. Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons. Elife 6, e28360 (2017).
Desai, B. S., Chadha, A. & Cook, B. The stum gene is essential for mechanical sensing in proprioceptive neurons. Science 343, 1256–1259 (2014).
He, L., Binari, R., Huang, J., Falo-Sanjuan, J. & Perrimon, N. In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer. Elife 8, e46181 (2019).
Simpson, J. H. & Looger, L. L. Functional imaging and optogenetics in Drosophila. Genetics 208, 1291–1309 (2018).
Luo, L., Callaway, E. M. & Svoboda, K. Genetic dissection of neural circuits: a decade of progress. Neuron 98, 256–281 (2018).
Bos, J. L. Epac: a new cAMP target and new avenues in cAMP research. Nat. Rev. Mol. Cell Biol. 4, 733–738 (2003).
Robichaux, W. G. & Cheng, X. Intracellular cAMP sensor EPAC: physiology, pathophysiology, and therapeutics development. Physiol. Rev. 98, 919–1053 (2018).
Yang, H. H. & St-Pierre, F. Genetically encoded voltage indicators: opportunities and challenges. J. Neurosci. 36, 9977–9989 (2016).
Bando, Y., Sakamoto, M., Kim, S., Ayzenshtat, I. & Yuste, R. Comparative evaluation of genetically encoded voltage indicators. Cell Rep. 26, 802–813.e4 (2019).
Fosque, B. F. et al. Labeling of active neural circuits in vivo with designed calcium integrators. Science 347, 755–760 (2015).
Grashoff, C. et al. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature 466, 263–266 (2010).
Krieg, M., Dunn, A. R. & Goodman, M. B. Mechanical control of the sense of touch by β-spectrin. Nat. Cell Biol. 16, 224–233 (2014).
Schmied, C. & Tomancak, P. Sample reparation and mounting of Drosophila embryos for multiview light sheet microscopy. Methods Mol. Biol. 1478, 189–202 (2016).
Buhlan, M., Ljaschenko, D., Scholz, N. & Langenhan, T. Figshare Raw Image Data. Available at https://doi.org/10.6084/m9.figshare.24024480

Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster

Supplementary information

推荐阅读