Elution of nasal lining fluid collected via nasosorption

Add 0.05% volume of Tween20 to PBS in a sterile environment to reduce cross-contamination

Store at 4°C until required

Thaw nasosorption device in cryotube on ice

Add 300µL of elution buffer to a labelled 2mL microcentrifuge tube and place on ice

Remove the synthetic absorptive matrix (SAM) from the nasosorption device using sterile forceps by tearing it from the handle and place in the microcentrifuge tube containing elution buffer

Vortex the microcentrifuge tube for 0h 0m 30s

Using a new pair of sterile forceps, remove the SAM from the elution buffer, and place into a spinX column on a new 2mL microcentrifuge tube

Add the elution buffer from the first tube onto the SAM in the spinX column

Centrifuge the SAM and spinX column at 16000x g,0h 0m 0s for 0h 30m 0s at 4°C

Discard the spinX column and SAM

Aliquot the eluate into labelled microtubes, and measure total protein concentration using the assay of your choice (Pierce BCA assay, Qubit protein assay etc)