Direct delivery and fast-treatedAgrobacteriumco-culture (Fast-TrACC) plant transformation methods forNicotiana benthamiana

Jon P. Cody, Michael F. Maher, Ryan A. Nasti, Colby G. Starker, James C. Chamness, Daniel F. Voytas

Published: 2022-10-16 DOI: 10.1038/s41596-022-00749-9

genome editing

Agrobacterium

Fast-TrACC

plant transformation

Nicotiana benthamiana

AI 解读

The GFP thermal shift assay for screening ligand and lipid interactions to solute carrier transporters

Co-fractionation–mass spectrometry to characterize native mitochondrial protein assemblies in mammalian neurons and brain

Simultaneously measuring the methylation of parent and daughter strands of replicated DNA at the single-molecule level by Hammer-seq

The Nodewalk assay to quantitate chromatin fiber interactomes in very small cell populations

Nondestructive production of exosomes loaded with ultrathin palladium nanosheets for targeted bio-orthogonal catalysis

An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells

Engineering SARS-CoV-2 using a reverse genetic system

cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Growing and making nano- and microcrystals

Composable microfluidic spinning platforms for facile production of biomimetic perfusable hydrogel microtubes

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There is an expanding need to modify plant genomes to create new plant germplasm that advances both basic and applied plant research. Most current methods for plant genome modification involve regenerating plants from genetically modified cells in tissue
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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Direct delivery and fast-treatedAgrobacteriumco-culture (Fast-TrACC) plant transformation methods forNicotiana benthamiana

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