DAB-quant

Sneh Patel, Sara Fridovich-Keil, Shauna Rasmussen, and Judith L Fridovich-Keil

Published: 2022-07-21 DOI: 10.17504/protocols.io.b93rr8m6

浏览 1167

Abstract

Here we describe DAB-quant, a new system that facilitates quantitation of large numbers of scanned tissue slides stained via immunohistochemistry with 3,3′-Diaminobenzidine (DAB). The python code, instructions, license, and a link to example scans for analysis are all available at:

https://github.com/sarafridov/DAB-quant

Steps

1.

Here we provide DAB-quant, a new system that facilitates quantitation of large numbers of scanned tissue slides stained via immunohistochemistry with 3,3′-Diaminobenzidine (DAB). The python code, instructions, license, and a link to example scans for analysis are all available at:

https://github.com/sarafridov/DAB-quant

推荐阅读

Nature Protocols

Protocols IO

Current Protocols

Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers

Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules

Three-dimensional human neural culture on a chip recapitulating neuroinflammation and neurodegeneration

A plate-based single-cell ATAC-seq workflow for fast and robust profiling of chromatin accessibility

Reproducible mass spectrometry data processing and compound annotation in MZmine 3

Production and use of antigen tetramers to study antigen-specific B cells

Probing the free-state solution behavior of drugs and their tendencies to self-aggregate into nano-entities

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

Design of metal-mediated protein assemblies via hydroxamic acid functionalities

Preparation of AIEgen-based near-infrared afterglow luminescence nanoprobes for tumor imaging and image-guided tumor resection

查看全部

扫码咨询