Control of DNA replication in vitro using a reversible replication barrier

Emma J. Vontalge, Tamar Kavlashvili, Steven N. Dahmen, Matthew T. Cranford, James M. Dewar

Published: 2024-04-09 DOI: 10.1038/s41596-024-00977-1

DNA replication

Reversible replication barrier

In vitro

Replication fork

Lac repressor

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References

Introduction
Materials
Procedure 1: preparation of materials
Procedure 2: experimental approaches
- In vitro DNA replication using LacR as a reversible replication barrier
- Separation of replication intermediates using native agarose gels
Procedure 3: analytical gels
Procedure 4: data acquisition and analysis
- Phosphorimaging
- ImageJ analysis
Troubleshooting
Timing
Anticipated results
Acknowledgements

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Control of DNA replication in vitro using a reversible replication barrier

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