Collections fromNature Protocols Published: 2023-12-19 DOI: 10.1038/s41596-023-00946-0 AI 解读 浏览 1058 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation Longitudinal interrogation of sympathetic neural circuits and hemodynamics in preclinical models Experimental modulation of physiological force application on leg joint neurons in intactDrosophila melanogaster Combining surface-accessible Ag and Au colloidal nanomaterials with SERS for in situ analysis of molecule–metal interactions in complex solution environments Generation of mouse–human chimeric embryos Labeling and tracking of immune cells in ex vivo human skin Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters Mutate-and-chemical-shift-fingerprint (MCSF) to characterize excited states in RNA using NMR spectroscopy Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing 查看全部 Single-cell ATAC sequencing Single-cell ATAC sequencing HPAP HIRN CRISPR puromycin Treatment and staining of iPSC-derived neurons for lysosomal phenotype analysis Workflow for proteomic analysis of purified lysosomes with or without damage Proteomic analysis Purified lysosomes ASAPCRN Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System Cell lysis Automation Proteomics Biomek Sample preparation Bacteria Human Embryonic Kidney Cells (HEK-293) Human Embryonic Kidney Cells (HEK-293) DMEM Drug treat Harvesting Electroporation of fluorescent antisense molecules into Bodo saltans and live imaging LC3-lipidation-assay ASAPCRN Sanger Tree of Life HMW DNA Extraction: Automated MagAttract v.2 HMW DNA extraction magnetic bead extraction MagAttract automated DNA extraction KingFisher solid phase reversible immobilisation reference genome long read sequencing High-Throughput RNA Extraction on Agilent Bravo RNA bead extraction Bravo RNA extraction magbinding beads magnetic bead extraction 查看全部 Purification of SRSF1 from E. coli for Biophysical and Biochemical Assays intrinsically disordered proteins NMR phase separation phosphorylation RNA splicing SR protein SRSF1 Surgical Procedure for Implantation of Opto-Array in Nonhuman Primates brain cortex inferior temporal cortex monkey optogenetics surgery Methods to Identify Immune Cells in Tissues With a Focus on Skin as a Model flow cytometry inflammation immune cells immunofluorescence immunohistochemistry multiplex skin skin inflammation skin immune cells A Human Neuron/Astrocyte Co-culture to Model Seeded and Spontaneous Intraneuronal Tau Aggregation astrocytes co-culture induced pluripotent stem cell neurons tau pathology Ploidy- and Purity-Adjusted Allele-Specific DNA Analysis Using CLONETv2 allele-specific analysis cancer genomics clonality ploidy purity Establishing a Microfluidic Tumor Slice Culture Platform to Study Drug Response breast cancer chemotherapy microfluidics organ-on-chip predictive assay Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2 SARS-CoV-2 COVID-19 COVID19 virus quantification plaque assay Production of Structured RNA Fragments by In Vitro Transcription and HPLC Purification biophysics HPLC RNA in vitro transcription sample preparation structural biology Mn2+-Phos-Tag Polyacrylamide for the Quantification of Protein Phosphorylation Levels electrophoresis phosphoproteomics phosphorylation Phos-tag western blotting Repli-seq Sample Preparation using Cell Sorting with Cell-Permeant Dyes cell cycle flow cytometry intact nuclei replication timing Repli-seq 查看全部
