Collections fromNature Protocols Published: 2023-12-19 DOI: 10.1038/s41596-023-00946-0 AI 解读 浏览 1112 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Inferring cellular and molecular processes in single-cell data with non-negative matrix factorization using Python, R and GenePattern Notebook implementations of CoGAPS Dual detection of chromatin accessibility and DNA methylation using ATAC-Me Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView Stabilization and structure determination of integral membrane proteins by termini restraining Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds Design, assembly, and characterization of membrane-spanning DNA nanopores Hyperosmolar blood–brain barrier opening using intra-arterial injection of hyperosmotic mannitol in mice under real-time MRI guidance Analysis framework and experimental design for evaluating synergy-driving gene expression Medulloblastoma and high-grade glioma organoids for drug screening, lineage tracing, co-culture and in vivo assay 查看全部 Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360 CHOP TMC Single Cell Multiome ATAC + Gene Expression HuBMAP Children's Hospital of Philadelphia TMC Cloning individual AAV capsid variants ASAPCRN Cell culture Targeted ExSeq -- In Situ Sequencing (Illumina Chemistry) expansion microscopy in situ sequencing expansion sequencing targeted ExSeq ExSeq spatial transcriptomics spatial omics spatially resolved transcriptomics Well-tempered Metadynamics protocol Synaptic immunohistochemistry - wholemount via acetone permeabilization Recombinant αS purification Protocol ASAPCRN protocols.io academic and non-profit contract Panicle Ratio Network: A high-throughput dynamic phenotype recognition model based on ultra-high-definition unmanned aerial vehicle images for rice panicle analysis in fields 查看全部 Prediction of Acute Hepatotoxicity With Human Pluripotent Stem Cell-Derived Hepatic Organoids cytochrome P450 drug testing hepatic organoid hepatotoxicity human pluripotent stem cell differentiation liver A Step-by-Step Guide for the Production of Recombinant Fluorescent TAT-HA-Tagged Proteins and their Transduction into Mammalian Cells DNA molecular assembly fluorescent protein fusion protein Ni-NTA-based protein purification protein transduction recombinant protein production tagged protein MHC II–EGFP Knock-in Mouse Model APCs light-sheet fluorescence microscopy lymphoid organs MHC II–EGFP knock-in mouse quantitative immunology SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup COVID19 COVID-19 ELISA protein expression SARS-CoV-2 serological assay Generation and Functional Characterization of Monocytes and Macrophages Derived from Human Induced Pluripotent Stem Cells differentiation functional characterization induced pluripotent stem cells macrophages monocytes Super-Resolution Stimulated Raman Scattering Microscopy with Graphical User Interface–Supported A-PoD deconvolution metabolic imaging stimulated Raman scattering super-resolution Protein Structural Modeling for Electron Microscopy Maps Using VESPER and MAINMAST cryo-EM alignment de novo protein modeling electron microscopy maps protein fitting protein structure Hepatitis E Virus: Isolation, Propagation, and Quantification HEV isolation propagation virus quantification Do more with Less: Improving High Parameter Cytometry Through Overnight Staining antibody incubation high-parameter cytometry optimization overnight staining spectral cytometry titration Isolation and Culturing of Primary Murine Chondroprogenitor Cells: A Mammalian Model of Chondrogenesis cartilage formation chondrogenesis limb bud micromass culture mouse embryo 查看全部
