Capture of the newly transcribed RNA interactome using click chemistry

Xiangpeng Guo, Muqddas Tariq, Yiwei Lai, Shahzina Kanwal, Yuan Lv, Xiwei Wang, Na Li, Mengling Jiang, Jin Meng, Jieyi Hu, Jianwen Yuan, Zhiwei Luo, Carl Ward, Giacomo Volpe, Dongye Wang, Menghui Yin, Baoming Qin, Biliang Zhang, Xichen Bao, Miguel A. Esteban

Published: 2021-10-24 DOI: 10.1038/s41596-021-00609-y

RNA-binding proteins

RNA interactome

click chemistry

5-ethynyluridine

affinity capture

AI 解读

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An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction

Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening

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Application of synthetic nucleoside analogues to capture newly transcribed RNAs has unveiled key features of RNA metabolism. Whether this approach could be adapted to isolate the RNA-bound proteome (RNA interactome) was, however, unexplored. We have devel
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ACKNOWLEDGMENTS

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Capture of the newly transcribed RNA interactome using click chemistry

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