Beetle rearing media
Yin-Tse Huang, Tina
Published: 2022-06-14 DOI: 10.17504/protocols.io.3byl4b62jvo5/v1
Abstract
ambrosia beetle rearing
Steps
1.
The recipe produces enough medium to fill approximately 18 culture tubes.(12cm)
First, add powder or solid
| A | B |
|---|---|
| component | amount |
| streptomycin | 0.175 g |
| Wesson's salt mixture | 0.625 g |
| yeast | 4 g |
| corn starch | 4 g |
| casein | 5 g |
| agar | 15 g |
| sucrose | 2.5 g |
| redbay sawdust | 100 g |
2.
Second, add liquid
| A | B |
|---|---|
| component | amount |
| wheat germ oil | 1.25ml |
| 95％ ethanol | 2.5ml |
| deionized water | 290ml |
3. 
All ingredients were mixed thoroughly, then put cotton plugs into the mouth of the filled tubes.
4.
The culture tubes was autoclaved at 121 °C for 30 min.
5. 
Put culture tubes to dry 2 days in oven at 55°C.
(cotton plugs don't remove)
