Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy

Nathalie Gaudreault, Benjamin W. Gregor, Mackenzie E. Coston, Ellen M. Adams, Joy Arakaki, Antoine Borensztejn, Thao P. Do, Margaret A. Fuqua, Amanda Haupt, Melissa C. Hendershott, Winnie Leung, Irina A. Mueller, Aditya Nath, Angelique M. Nelson, Susanne M. Rafelski, Emmanuel E. Sanchez, Madison J. Swain-Bowden, W. Joyce Tang, Derek J. Thirstrup, Winfried Wiegraebe, Brian P. Whitney, Calysta Yan, Ruwanthi N. Gunawardane

Published: 2023-12-12 DOI: 10.1038/s41596-023-00912-w

deep learning

automation

hiPS cells

3D microscopy

image datasets

AI 解读

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Introduction
Materials
Procedure 1: automated Matrigel coating (workflow 3)
Procedure 2: manual cell thawing and seeding
Procedure 3: automated plate maintenance (workflow 1)
Procedure 4: automated cell passaging (workflow 2)
Troubleshooting
Timing
Anticipated results
Acknowledgements

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Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy

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